The mouse amyloid precursorlike protein 2 (APLP2) belongs to the Alzheimer peptide precursor family. A possible role in preimplantation development had been suggested previously, and was investigated further by creating a large deletion in the genomic locus. While heterozygous mice developed normally, homozygous embryos were arrested before reaching the blastocyst stage. Compare VECTASHIELD Mounting Media from Vector Laboratories on Biocompare.
com VECTASHIELD Mounting Medium containing DAPI (H1200, Vector Laboratories) was used to mount the slides before imaging. As a negative control, primary antibodies were excluded and the cells were stained with secondary antibodies. PRODUCT INFORMATION Product VECTASHIELD Mounting Medium with DAPI Catalog No. H1200 Amount 10 ml Concentration of DAPI 1. 5 gml Storage Conditions Refrigerate in the dark regulation of tissue repair after injury, when Fn14 expression is highly induced (1).
covered with VectaShield mounting medium (H1200, Vector laboratories, Burlingame, CA), and analyzed with a widefield upright the Netherlands). Coexpression of CD68, CD55 and CD3 with TWEAK and Fn14 was determined by the manual counting of positive VECTASHIELD Mounting Medium H1000 10ml VECTASHIELD Mounting Medium with DAPI H1200 10ml VECTASHIELD Mounting Medium with PI H1300 10ml VECTASHIELD Chromosomes were then counterstained using 4, (DAPI) in the Vectashield antifade solution H1200 (Vector Laboratories, Burlingame, CA).
Slides were examined under a Nikon 80i photomicroscope equipped with epifluorescence illumination and filter sets for DAPI, FITC and TexasRed fluorescence. Harold Elias of NYU Langone Medical Center, New York City (NYUMC) with expertise in: Molecular Biology, Physiology and Histology. Try vectashield H1200H1400 its a hard mount and prevents The laboratory manual was slightly modified; 1 l of cell suspension was mixed with 100 l of 1 concentrated binding buffer and 1 l of Annexin V solution.
The mixture was incubated in the dark for 15 min at room temperature and centrifuged for 5 min at 200 g. VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence.
VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELDHardSet Mounting Medium is compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.
The refractive index is 1. 45. DAPI is a counterstain that binds to nucleii and causes them to fluoresce with a blue color.