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Nutrient broth composition difco manual pdf

Difco Nutrient Broth. Dehydrated Appearance: Medium tan, freeflowing, homogeneous. Solution: 0. 8 solution, soluble in purified water. Solution is light to medium amber, clear.

Prepared Appearance: Light to medium amber, clear. Reaction of 0. 8 Solution at 25C: pH 6. 8 0. 2. Cultural Response. Difco Nutrient Broth. Prepare NUTRIENT BROTH (7146) Intended Use Nutrient Broth is used for the cultivation of a wide variety of microorganisms in a laboratory setting.

Nutrient Bacteriological Analytical Manual. 1995. 8th ed. Association of Official Analytical Chemists. Gaithersburg, MD. Technical Information Difco& BBL Manual, 2nd Edition WL Nutrient Medium WL Nutrient Broth WL Differential Medium Intended Use WL Nutrient Medium and WL Nutrient Broth Difco Nutrient Broth Approximate Formula Per Liter Beef Extract.

3. 0 g Peptone. 5. 0 g Adjusted andor supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Nutrient Broth is one of several nonselective media useful in routine cultivation of microorganisms. Nutrient Broth No. 2, for microbiology; Synonym: Standard Nutrient Broth; find SigmaAldrich MSDS, related peerreviewed papers, technical documents, similar products& more at SigmaAldrich.

MANUAL DIFCO Download as PDF File (. pdf), Text File (. txt) or read online. Difco Nutrient Broth Prepare the medium per label directions. Inoculate and incubate at 35 2C for 1824 hours. ORGANISM ATCC INOCULUM CFU RECOVERY Escherichia coli Good Staphylococcus aureus Good Nutrient Gelatin Intended Use Nutrient Gelatin is used for the detection of gelatin